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Antibodies
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anti-Transforming Growth Factor, alpha (TGFA) antibody
Transforming Growth Factor, alpha (TGFA) antibody
| Antigen |
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| Synonyms |
TFGA, wa1, wa-1, TGFAA, RATTGFAA, TGFA, TGF-A, TGF alpha, LOC100009482, NV15606, DKFZp469C1513 |
| Clonality |
Monoclonal (MF9-TG86) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (20), Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), ELISA (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (IHC) (5), Immunoprecipitation (IP) (5), Functional Studies (Func) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Blocking Antibody (Inhibition) (1), Immunoelectron Microscopy (IEM) (1), Neutralization (Neut) (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN125456 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
284.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Transforming Growth Factor-alpha (TGFa)
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Immunogen
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BALB/C mice were injected with synthetic peptides from rat (MF9) and human (TG86) TGF alpha.
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Format
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Ig-fraction
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Isotype
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IgG1, IgM (Matching secondary antibodies)
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Clone
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MF9-TG86
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Specificity
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This antibody reacts with a 6 kD protein known as TGF alpha. TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of receptor and stimulates cell proliferation. TGF alpha plays role in tumor initiation by inducing the reversible transformed phenotype.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Anterior Pituitary. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cytokines, Growth Factors, Receptors, Immunology, Inflammation
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Transforming Growth Factor, alpha (TGFA)", type "Antibodies"
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Hosts
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Rabbit (36), Sheep (7), Mouse (4), Goat (1)
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Reactivities
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Human (42), Rat (Rattus) (28), Mouse (Murine) (23), Cow (Bovine) (21), Dog (Canine) (19), Horse (Equine) (18), Pig (Porcine) (18), Rabbit (18), Guinea Pig (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (20), Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), ELISA (11), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (IHC) (5), Immunoprecipitation (IP) (5), Functional Studies (Func) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Blocking Antibody (Inhibition) (1), Immunoelectron Microscopy (IEM) (1), Neutralization (Neut) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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Biotin (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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