Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody

Antigen: Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4)

Synonyms: LeX, CD15, ELFT, FCT3A, FUTIV, SSEA-1, FUC-TIV

Clonality: Monoclonal (MMA)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125467

Additional Information

Alternative name: CD15

Immunogen: BALB/C mice were injected with U937 histiocytic cell line.

Format: Ig-fraction

Isotype: IgM kappa  (Matching secondary antibodies)

Clone: MMA

Specificity: This antibody reacts with a 220 kD protein. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosionphils and to a lesser degree on monocytes. CD15 is also expressed in Reed-Sternberg cells and some epithelial cells. CD15 is very useful in the identification of Hodgkin

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human Hodgkins lymphoma stained with CD15, Granulocyte Assoc. Antigen antibody