Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb) antibody

Antigen: Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated serine Esterase 1) (Gzmb)

Synonyms: HLP, CCPI, CGL1, CSPB, SECT, CGL-1, CSP-B, CTLA1, CTSGL1, GZB, CCP1, Ctla1, Ctla-1, AI553453, CCP-1/C11, RNKP-1, MGC156565, GZMB

Clonality: Monoclonal (GZB01)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125468

Additional Information

Alternative name: Granzyme B

Immunogen: BALB/C mice were injected with recombinant protein encoding the N-terminus of the mature granzyme B.

Format: Supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: GZB01

Specificity: This antibody reacts with a 32 kD protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned. Human granzymes B is involved in target cell apoptosis during lymphocyte-mediated cytotoxicity. This antibody is useful in the localization of granzyme B containing lytic granules and for the characterization of activated CTLs or NK cells.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Hodgkin

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes, Metabolism

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human Hodgkins lymphoma stained with Granzyme B antibody