P300/CBP antibody

Antigen: P300/CBP

Synonyms: CBP, KAT3A, AW558298, CBP/p300, p300/CBP, CBP_, Crbp, anon-WO0147981.11, anon-WO03040301.89, dCBP, dmCBP, nej CBP, p300, DmelCG15319, CG15319

Clonality: Monoclonal (NM11)

Host: Mouse

Reactivity: Human, Monkey, Mink, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125474

Additional Information

Immunogen: BALB/C mice were immunized with E1A affinity purified p300 protein.

Format: Purified

Isotype: IgG2b kappa  (Matching secondary antibodies)

Clone: NM11

Specificity: This antibody stains with a 300 kD protein. P300 and CBP (CREB-binding protein) are homologous to each other. P300 is transcriptional adapter protein, which is characterized by three cysteine and histidine-rich regions. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kD nuclear protein designated CBP. This antibody crossreacts with monkey, mink, mouse and rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Transcription Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union