Myeloid Cell Leukemia (Mcl-1) antibody

Antigen: Myeloid Cell Leukemia (Mcl-1)

Clonality: Monoclonal (RC13)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125476

Additional Information

Immunogen: BALB/C mice were immunized with recombinant human Mcl-1 protein corresponding to amino acid 1-327.

Format: Purified

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: RC13

Specificity: This antibody stains with a 42 kD protein. Myeloid cell leukema-1 (Mcl-1) is a member of bcl-2 family, which blocks programmed cell death. The carboxy terminal of Mcl-1 and bcl-2 share significant sequence homology. Like bcl-2, Mcl-1 protein can interact with Bax, inhibiting BAX- induced cell death and promoting cell viability. Mcl-1 stains epithelial cells in a variety of tissues including prostate, breast, endometrium, epidermis, stomach, small intestine, colon and respiratory tract.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Tonsil. Cellular localization: Nuclear, occasionally cytoplasmic Other Applications: Immunoprecipitation, Western blotting (1:100-500).

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union