S-100 Protein (alpha chain) antibody

Antigen: S-100 Protein (alpha chain)

Clonality: Monoclonal (4C4-9)

Host: Mouse

Reactivity: Cow (Bovine)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125477

Additional Information

Alternative name: S-100 Protein

Immunogen: BALB/C mice were immunized with purified bovine brain S-100 protein

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 4C4-9

Specificity: This antibody stains with S-100 protein. S-100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S-100A and S-100B proteins are two members of the S-100 family. S-100A is composed of an alpha and beta chain whereas S-100B is composed of two beta chains. This antibody stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. This antibody crossreacts with human, cow, mouse and rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin fixed tissues. No treatment is required. Positive Control: Melanoma. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human melanoma stained with S-100 antibody

Publications

Publications: Barrett, Scully: "S100 protein in oral biology and pathology." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 23, Issue 10, pp. 433-40, 1995 (PubMed).

Matsushima, Mori, Adachi et al.: "S100 protein positive human breast carcinomas: an immunohistochemical study." in: Journal of surgical oncology, Vol. 55, Issue 2, pp. 108-13, 1994 (PubMed).

McLaren, Cossar: "The immunohistochemical localization of S100 in the diagnosis of papillary carcinoma of the thyroid." in: Human pathology, Vol. 27, Issue 7, pp. 633-6, 1996 (PubMed).

Joshi, Lee, Pedersen et al.: "The role of immunocytochemical markers in the differential diagnosis of proliferative and neoplastic lesions of the breast." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 9, Issue 1, pp. 57-62, 1996 (PubMed).