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Antibodies
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anti-E2F Transcription Factor 1 (E2F1) antibody
E2F Transcription Factor 1 (E2F1) antibody
| Antigen |
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| Synonyms |
RBP3, E2F-1, RBAP1, RBBP3, KIAA4009, mKIAA4009, E2F1, xE2F |
| Clonality |
Monoclonal (KH95) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125481 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
296.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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E2F-1 Transcription Factor
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Immunogen
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BALB/C mice were immunized with a recombinant Human E2F-1 protein.
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Format
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Purified
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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KH95
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Specificity
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This antibody reacts with a protein of 60 kD. E2F
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil, Breast carcinoma. Cellular localization: Nuclear
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified ascites containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Transcription Factors
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "E2F Transcription Factor 1 (E2F1)", type "Antibodies"
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Hosts
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Rabbit (13), Mouse (1)
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Reactivities
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Human (14), Chimpanzee (1), Cow (Bovine) (1), Monkey (1), Mouse (Murine) (1), Rat (Rattus) (1)
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Applications
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Western Blotting (WB) (9), ELISA (7), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Dot Blot (Dot) (2), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (1)
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Epitopes
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pSer364 (4), pS364 (2), H357 (1), S332 (1), S337 (1), pS332 (1), pS337 (1), pSer332 (1), pSer337 (1)
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