Minichromosome Maintenance Complex Component 2 (MCM2) antibody

Antigen: Minichromosome Maintenance Complex Component 2 (MCM2)

Synonyms: BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN, KIAA0030, MGC10606, cb737, chunp6905, mcm2, MGC89529

Clonality: Monoclonal (CRCT2-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125483

Additional Information

Alternative name: MCM2

Immunogen: BALB/C mice were immunized with a Bacterially expressed human MCM2 protein.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: CRCT2-1

Specificity: This antibody with a protein of 120- 125 kD. Mini Chromosome Maintainance Protein-2 (MCM2) is involved in the control of DNA replication. MCM proteins form a complex, which exhibits ATPase and helicase activities. In G0 phase levels of MCM2 and MCM5 are lower than that of MCM7 and 3 but the level increases as the cell enters G1/s phase of the cell cycle.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union