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Antibodies
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anti-Minichromosome Maintenance Complex Component 5 (MCM5) antibody
Minichromosome Maintenance Complex Component 5 (MCM5) antibody
| Antigen |
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| Synonyms |
CDC46, MGC5315, P1-CDC46, Cdc46, Mcmd5, mCD46, mCDC46, AA617332, AI324988, AL033333, mcm5-b, MGC68977, mcm5, MGC97563, 23.m06024 |
| Clonality |
Monoclonal (CRCT5-1) |
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Host
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Reactivity
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Human (38), Mouse (Murine) (25), Rat (Rattus) (21), Cow (Bovine) (18), Horse (Equine) (18), Dog (Canine) (17), Rabbit (17), Pig (Porcine) (3), Chicken (1), Sheep (Ovine) (1), Xenopus laevis (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (21), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunoprecipitation (IP) (6), ELISA (4), Immunohistochemistry (IHC) (4), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125484 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
286.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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MCM5
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Immunogen
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BALB/C mice were immunized with a Bacterially expressed human MCM5 protein.
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Format
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Purified
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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CRCT5-1
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Specificity
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This antibody with a protein of 90 kD. Mini Chromosome Maintainance Protein-5 (MCM5) is involved in the control of DNA replication. MCM proteins form a complex, which exhibits ATPase and helicase activities. In G0 phase levels of MCM2 and MCM5 are lower than that of MCM7 and 3 but the level increases as the cell enters G1/s phase of the cell cycle.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Cervical carcinoma. Cellular localization: Nuclear
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified ascites containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Minichromosome Maintenance Complex Component 5 (MCM5)", type "Antibodies"
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Hosts
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Rabbit (32), Mouse (10)
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Reactivities
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Human (38), Mouse (Murine) (25), Rat (Rattus) (21), Cow (Bovine) (18), Horse (Equine) (18), Dog (Canine) (17), Rabbit (17), Pig (Porcine) (3), Chicken (1), Sheep (Ovine) (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (21), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunoprecipitation (IP) (6), ELISA (4), Immunohistochemistry (IHC) (4), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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