Add to Basket
Order hotline:
 |
+1 404 474 4654 |
 |
+1 888 205 9894 (TF) |
Paxillin (PXN) antibody
| Antigen | Paxillin (PXN) |
| Synonyms | FLJ16691, Pax, AW108311, AW123232, CG18061, CG18576, CT40481, CT42454, DPaxillin, DPxn, DPxn37, Dpax, PDLP, DmelCG31794, CG31794, LOC397826, wu:fw71f12, PXN, DKFZp469J2119, LOC100220858 |
| Clonality | Monoclonal (5H11) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|
| Catalog no. | ABIN125485 |
| Quantity | 500ul (100ug/ml approximately) (Variants) |
| Price | 213.00 $ Plus shipping costs $35.00 |
| Shipping to |
|
| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | Paxillin |
| Immunogen | BALB/C mice were immunized with purified full-length recombinant human paxillin protein. |
| Format | Purified |
| Isotype | IgG1 kappa (Matching secondary antibodies) |
| Clone | 5H11 |
| Specificity | This antibody with a protein of 68 kD. Paxillin is a focal adhesion protein and a substrate for several tyrosine kinase such as src, FAK and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation. This antibody cross reacts with rat. |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Cell membrane, cytoplasmic |
| Concentration | 100ug/ml approximately |
| Buffer | This antibody is supplied as purified ascites containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Extracellular Matrix |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Alternatives
Alternatives for antigen "Paxillin (PXN)", type "Antibodies"
| Hosts | Rabbit (86), Mouse (2) |
| Reactivities | Human (89), Mouse (Murine) (48), Rat (Rattus) (48), Chicken (2), Dog (Canine) (2), Cow (Bovine) (1), Horse (Equine) (1), Monkey (1), Pig (Porcine) (1), Xenopus laevis (1) |
| Applications | Western Blotting (WB) (67), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (26), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), ELISA (10), Enzyme Immunoassay (EIA) (5), Immunoprecipitation (IP) (4), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | pTyr118 (10), pTyr31 (8), pTyr88 (6), C-Term (2), Center (2), N-Term (1), Y118 (1), pSer178 (1) |
