JAK3 antibody

Antigen: JAK3

Clonality: Monoclonal (A1-14-16)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125486

Additional Information

Immunogen: BALB/C mice were immunized with recombinant protein encoding C-terminus corresponding to amino acid 1076

Format: Purified

Isotype: IgG2a kappa  (Matching secondary antibodies)

Clone: A1-14-16

Specificity: This antibody with a protein of 115 kD. JAK3 is a member of the Janus family of protein tyrosine kinases along with JAK1, JAK2 and Tyk2. JAK3 is activated in response to IL-3 and IL-4 in T cells and myeloid cells. JAK3 activation requires the serine-rich membrane-proximal region of the IL-2 receptor beta-chain. Expression of JAK3 is restricted to NK and T cells. This antibody cross reacts with mouse.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Cell membrane, cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Signaling, Cytokines, Receptors, Kinases/Phosphatases

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union