HPV16 antibody

Antigen: HPV16

Clonality: Monoclonal (CAMVIR-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125491

Additional Information

Alternative name: HPV 16

Immunogen: BALB/C mice were immunized with recombinant HPV-16 L1 protein.

Isotype: IgG2a  (Matching secondary antibodies)

Clone: CAMVIR-1

Specificity: This antibody stains with HPV type 16. L1 protein is a major capsid protein of HPV type 16. HPV 6 and 11 have been associated with relatively benign diseases such as genital warts but type 16 and 18 are strongly associated with cervical, vaginal and vulvar malignancies.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: HPV type 16 infected cervix. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Virology

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union