Herpes Virus 8 (HHV8) antibody

Antigen: Herpes Virus 8 (HHV8)

Clonality: Monoclonal (LN53)

Host: Rat

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125492

Additional Information

Immunogen: Recombinant protein corresponding to the latent nuclear antigen-1 molecule of HHV8.

Format: Tissue culture supernatant (purified)

Isotype: IgG2c  (Matching secondary antibodies)

Clone: LN53

Specificity: This antibody stains with ORF-73 of human Herpesvirus 8 (HHV8). HHV8 is the likely etiological agent of Kaposi’s sarcoma (KS). HHV8 encodes a latent nuclear antigen (LNA), which is the product of the viral gene orf 73. LNA is capable of forming a complex with retinoblastoma susceptibility gene product, which may be related to its oncogenic activity.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Kaposi

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified tissue culture containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Virology

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Cultured cells infected with HHV8 stained with HHV8 antibody