Synaptophysin (SYP) antibody

Antigen: Synaptophysin (SYP)

Synonyms: Syn, p38, AI848995, A230093K24Rik, Syp1, syp-A, xsypi, MGC86277, syp-B, xsypii, MGC86267

Clonality: Monoclonal (SYP02)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125495

Additional Information

Alternative name: Synaptophysin

Immunogen: BALB/C mice were injected with a synthetic peptide encoding a region near the c-terminal end of the synaptophysin protein.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: SYP02

Specificity: This antibody is specific to 38 kD protein. Synaptophysin is an glycoprotein occurring in presynaptic vesicles of neurons in the brain, spinal cord, retina, vesicles of adrenal medulla and in neuromuscular junctions. This antibody reacts with neuroendocrine neoplasms of neural as well as epithelial types.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreas. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human pancreas stained with Synaptophysin antibody