Insulin (INS) antibody
| Antigen |
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| Synonyms |
ILPR, IRDN, INS, MGC159719, ins1, xins, ins1-a, Insulin |
| Clonality |
Monoclonal (E2-E3) |
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Host
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Reactivity
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 Alternatives Human, Cow (Bovine), Pig (Porcine), Rabbit, Rat (Rattus)
Human (159), Pig (Porcine) (91), Rat (Rattus) (58), Mouse (Murine) (45), Cow (Bovine) (40), Rabbit (28), Horse (Equine) (22), Dog (Canine) (9), Chicken (3), Guinea Pig (3), Sheep (Ovine) (3), Cat (Feline) (2)
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Application
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Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Immunofluorescence (IF) (77), Flow Cytometry (FACS) (71), ELISA (51), Enzyme Immunoassay (EIA) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Radioimmunoassay (RIA) (25), Western Blotting (WB) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Immunohistochemistry (IHC) (17), Immunoprecipitation (IP) (13), ELISA (Capture) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Functional Studies (Func) (4), Immunocytochemistry (ICC) (4), Immunoelectron Microscopy (IEM) (3)
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| Catalog no. |
ABIN125501 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
163.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Insulin
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Immunogen
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Swine insulin.
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Format
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Ascites (prediluted)
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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E2-E3
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Specificity
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Insulin is a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin is a 51 amino acid polypeptide composed of A and B chains connected through the C-peptide. Insulin promotes glycogen storage, formation of triglycerides and synthesis of protein and nucleic acids. Cross reaction has been observed with insulin containing cells in fixed sections of pancreas from human, cow, pig, rabbit and rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. No pretreatment is required for paraffin sections. Positive Control: Pancreas. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as diluted ascites containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Diabetes, Hormones
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Insulin (INS)", type "Antibodies"
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Hosts
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Rabbit (89), Mouse (82), Guinea Pig (8), Sheep (4), Chicken (1)
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Reactivities
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Human (159), Pig (Porcine) (91), Rat (Rattus) (58), Mouse (Murine) (45), Cow (Bovine) (40), Rabbit (28), Horse (Equine) (22), Dog (Canine) (9), Chicken (3), Guinea Pig (3), Sheep (Ovine) (3), Cat (Feline) (2)
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Applications
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Immunofluorescence (IF) (77), Flow Cytometry (FACS) (71), ELISA (51), Enzyme Immunoassay (EIA) (34), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (34), Radioimmunoassay (RIA) (25), Western Blotting (WB) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (21), Immunohistochemistry (IHC) (17), Immunoprecipitation (IP) (13), ELISA (Capture) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Functional Studies (Func) (4), Immunocytochemistry (ICC) (4), Immunoelectron Microscopy (IEM) (3)
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Conjugates
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Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Biotin (6), PE,Cy5.5 (6), HRP (5), FITC (4), Alexa Flour 488 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy7 (3), RBITC (3)
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Epitopes
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beta chain (3), AA 57-87 (2), C-Term (2), Center (1)
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