Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) antibody

Antigen: Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2)

Synonyms: COX2, COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, GRIPGHS, Cox-2, Pghs2, TIS10, Cox2, PTGS2, Ptgs2, cox-2, cox2, MGC80431, phs-2, hcox-2, pgg/hs, pghs-2, MGC97821, PGHS2

Clonality: Monoclonal (4H12)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125506

Additional Information

Alternative name: Cyclooxygenase-2 (COX-2)

Immunogen: Prokaryotic recombinant protein corresponding to amino acids 30 to 163 of the N-terminus of the human COX-2 molecule.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 4H12

Specificity: COX-2 is a mitogen-inducible form of Cyclooxygenase, which is expressed in response to various inflammatory stimuli. COX-2 staining increases in the more differentiated, suprabasal keratinocytes of normal skin. COX-2 is expressed in epithelial and interstitial cells of adenomatous polyps and Colon adenocarcinomas.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Colon Ca. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes, Inflammation

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union