Thyroid Peroxidase (TPO) antibody

Antigen: Thyroid Peroxidase (TPO)

Synonyms: MSA, TPX, TDH2A, TPO, msa, tpx, xtpo, LOC100218312

Clonality: Monoclonal (MoAB47)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125510

Additional Information

Immunogen: Human Thyroid Peroxidase (hTPO) purified from thyroid microsomes by immunoaffinity chromatography.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: MoAB47

Specificity: Thyroid Peroxidase (TPO) is a transmembrane protein of 107kD containing a heme prosthetic group and it is present as dimmer on the apical surface of the thyroid follicular cells. TPO is the primary enzyme involved in thyroid hormone synthesis. Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. This antibody may be useful aid in the differentiation between the benign and malignant thyroid tumors.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 1mM EDTA buffer, pH 9.0 must be performed prior to the immunostaining. Positive Control: Thyroid. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes, Metabolism

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union