O6-Methylguanine-DNA-Methyltransferase (MGMT) antibody

Antigen: O6-Methylguanine-DNA-Methyltransferase (MGMT)

Synonyms: MGMT

Clonality: Monoclonal (MT3-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125515

Additional Information

Immunogen: Recombinant human MGMT protein.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: MT3-1

Specificity: O6-Methylguanine-DNA-Methyltransferase (MGMT) is a 22 kD human DNA repair protein that removes O6-alkylguanine DNA adducts. MGMT acts as a suicide acceptor protein repairing the DNA by accepting alkyl groups and consequently inactivating itself. This antibody stains all mantle zone lymphocytes and 50%of germinal center lymphocytes. Basaloid epithelial cells of tonsil squamous mucosa were also stained positive with this antibody.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10 mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear and cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union