alpha-Methylacyl-CoA Racemase (AMACR) antibody

Antigen: alpha-Methylacyl-CoA Racemase (AMACR)

Synonyms: RACE, CBAS4, Macr1, Da1-8, Marc1, AMACR, DKFZp469O1232, amacr, MGC89832

Clonality: Monoclonal (4A4)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125518

Additional Information

Alternative name: PIN Cocktail P504S+p63

Immunogen: A recombinant protein derived from amino acid 1-205 of human p63. A synthetic human AMACR peptide.

Format: Supernatant + Rb / Ig Fraction

Isotype: IgG2a  (Matching secondary antibodies)

Clone: 4A4

Specificity: P504S has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of P504S protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. P504S can be used as a positive marker for PIN. The p63 gene is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkable divergent abilities to transactivate p53 reporter genes and induce apoptosis. The combination of P504S and p63 (PIN) may be extremely useful for diagnosing prostatic intraepithelial neoplasia (PIN). P504S stains cytoplasm in prostatic adenocarcinomas and atypical hyperplasia and p63 stains nuclei in normal and benign prostate glands.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Prostate carcinoma. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as a cocktail of tissue culture supernatant (p63) and purified immunoglobulin fraction of rabbit antiserum (P504S) containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human prostate carcinoma stained with PIN Cocktail antibody