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Antibodies
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anti-MutS Homolog 6 (E. Coli) (MSH6) antibody
MutS Homolog 6 (E. Coli) (MSH6) antibody
| Antigen |
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| Synonyms |
GTBP, HSAP, HNPCC5, cb326, wu:fb36c12, MSH6, LOC100227810 |
| Clonality |
Monoclonal (44) |
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Host
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Reactivity
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 Alternatives Human, Mouse (Murine), Rat (Rattus), Dog (Canine)
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Application
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Western Blotting (WB) (26), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), ELISA (12), Immunoprecipitation (IP) (9), Enzyme Immunoassay (EIA) (3), Gel Shift (GS) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Electrophoretic Mobility-Shift Assay (EMSA) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125520 |
| Quantity |
500ul (100ug/ml approximately) (Variants) |
| Price |
275.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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MSH6
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Immunogen
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Synthetic human MSH6 peptide.
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Format
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Ig-fraction
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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44
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Specificity
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MSH6 is a heterodimer of MSH2 and binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. This antibody cross reacts with mouse, rat and dog.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Positive Control: Tonsil, colon carcinoma. Cellular localization: Nuclear Other Applications: immunoprecipitation and Western blotting (1:100-500).
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Concentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as affinity purified immunoglobulin liquid containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "MutS Homolog 6 (E. Coli) (MSH6)", type "Antibodies"
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Hosts
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Rabbit (34), Mouse (21), Goat (2)
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Reactivities
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Human (53), Mouse (Murine) (24), Rat (Rattus) (22), Dog (Canine) (5), Rabbit (2)
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Applications
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Western Blotting (WB) (26), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), ELISA (12), Immunoprecipitation (IP) (9), Enzyme Immunoassay (EIA) (3), Gel Shift (GS) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Electrophoretic Mobility-Shift Assay (EMSA) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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