Wilms Tumor 1 (WT1) antibody

Antigen: Wilms Tumor 1 (WT1)

Synonyms: GUD, WAGR, WT33, WIT-2, WT1, WT, wt1-A, xWT1, CWT1, WT-1, wt1, MGC152697, zgc:152697, si:ch211-160j6.3, gud, wagr, wt-1, wt33, xwt1, wit-2

Clonality: Monoclonal (6F-H2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125526

Additional Information

Alternative name: Wilms Tumor 1 Protein (WT1)

Immunogen: BALB/C mice were injected with truncated human WT1 protein corresponding to N-terminal amino acids 1-181.

Isotype: IgG1  (Matching secondary antibodies)

Clone: 6F-H2

Specificity: Wilms' Tumor (WT1), is a gene involved in the induction of Wilms

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Wilms

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing BSA and sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union