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Tumor Necrosis Factor (TNF) antibody

Antigen

Tumor Necrosis Factor (TNF)
Synonyms
DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF,  ... show more
Clonality Monoclonal (28401-11)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125547
Quantity 500ul  (100ug/ml approximately)  (Variants)
Price 255.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name Tumor Necrosis Factor alpha (TNF alpha)
Immunogen Purified recombinant human tumor necrosis factor-alpha.
Isotype IgG1  (Matching secondary antibodies)
Clone 28401-11
Specificity Tumor necrosis factor-alpha (TNF-alpha), also called cachectin, is a member of the TNF superfamily of cytokines. TNF-alpha is expressed as a 26 kD membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kD monomer, which forms homotrimers in circulation. The antibody does not cross-react with recombinant human TNF-alpha, TNF sRI, TNF sRII, nor with recombinant mouse TNF-alpha.
Synonyms DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the use. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Spleen. Cellular localization: Cell membrane, Extracellular
Concentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Tumor Necrosis Factor (TNF)", type "Antibodies"
Hosts Mouse (185), Rabbit (167), Rat (49), Goat (22), Hamster (11), Sheep (5), Chicken (4), Armenian Hamster (2)
Reactivities Human (291), Mouse (Murine) (131), Rat (Rattus) (75), Pig (Porcine) (45), Horse (Equine) (42), Dog (Canine) (37), Cow (Bovine) (31), Rabbit (23), Chicken (6), Sheep (Ovine) (5), Baboon (4), Monkey (4), Guinea Pig (2)
Applications Western Blotting (WB) (206), ELISA (177), Flow Cytometry (FACS) (159), Enzyme Immunoassay (EIA) (99), Immunofluorescence (IF) (60), Immunohistochemistry (Frozen Sections) (IHC (fro)) (50), Functional Studies (Func) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (44), Neutralization (Neut) (34), Radioimmunoassay (RIA) (34), Immunoprecipitation (IP) (31), Immunohistochemistry (IHC) (24), Blocking Antibody (Inhibition) (9), ELISPOT (8), ELISA (Capture) (4), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Intracellular Staining (IS) (4), Immunoelectron Microscopy (IEM) (3), Transfected cell culture (TCC) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1)
Conjugates Biotin (52), FITC (20), PE (12), Cy5.5 (5), HRP (5), RPE (5), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Cy7,PE (4), PE,Cy5.5 (4), APC (3), Cy3 (3), Cy5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy7 (3), RBITC (3), Alexa Flour 488 (2)