Chromogranin A (CHGA) antibody

Antigen: Chromogranin A (CHGA)

Synonyms: CGA, MGC105435, fj05f09, wu:fj05f09, zgc:101749, si:dkey-177p2.2, CHGA

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125552

Additional Information

Alternative name: Chromogranin A

Immunogen: C-terminal half of chromogranin A was purified from urine of patients with carcinoid syndrome.

Format: Ig-fraction

Specificity: This antibody reacts with human chromogranin A in the neuroendocrine cells and neural tissues. It does not recognizes the epitopes located on the N-terminal half of the chromogranin A molecule.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreas. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human chromogranin A containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer, Neurotransmitters

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human pancreas stained with Chromogranin A antibody