Helicobacter Pylori (H. pylori) antibody

Antigen: Helicobacter Pylori (H. pylori)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125557

Additional Information

Alternative name: Helicobacter pylori

Immunogen: Total lysate of H. pylori were injected to the rabbit.

Format: Ig-fraction

Specificity: This antibody reacts with the whole H. pylori. The antibody stains the bacteria present on the surface of the epithelium or in the cytoplasm of the epithelial cells. H. pylori is known to cause peptic ulcer and chronic gastritis in human.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Infected stomach. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human H. pylori containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Bacteria

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human stomach infected with H. pylori and stained with H. pylori antibody