Poly ADP-Ribose Polymerase (PARP) antibody

Antigen: Poly ADP-Ribose Polymerase (PARP)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125587

Additional Information

Immunogen: A synthetic peptide from the N-terminal region of human PARP.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 116 kD protein, known as PARP. Proteolysis of PARP to its stable 85 kD fragment is an early marker of apoptosis and is mediated by Caspase (CPP32) protein. PARP uses nicotinamide adenine dinucleotide (oxidized form) as a substrate to catalyze the transfer of ADP-ribose to a variety of nuclear acceptors. This antibody cross reacts with human, mouse and rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear Other Applications: Western Blotting

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human PARP containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union