Cullin 3 (CUL3) antibody

Antigen: Cullin 3 (CUL3)

Synonyms: CUL-3, Cullin-3, cul3, cul3a, CUL-3-A, MGC79108, Cullin-3-A, cul3b, CUL-3-B, MGC80402, Cullin-3-B, AI467304, AW146203, mKIAA0617, MGC189292, wu:fc37e01, CUL3, ARABIDOPSIS THALIANA CULLIN 3, ARABIDOPSIS THALIANA CULLIN 3A, ATCUL3, ATCUL3A, CUL3A, CULLIN 3, CULLIN 3A, T24P13.25

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Catalog no.: ABIN125591

Additional Information

Alternative name: Cullin-3 (CUL-3)

Immunogen: A peptide derived from the N-terminus region of the human Cullin-3.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 85kD protein, known as Cullin-3. Cullin-3 is a member of the family of human cullin genes (CUL-1, -2, -3, -4a, -4b and 5) homologous to the S. cerevisiae cdc53 gene. Cullin-3 forms a tight complex with cyclin E and targets it for ubiquitination, which leads to its degradation by proteosomes. This antibody cross reacts with mouse.

Synonyms: CUL-3, Cullin-3, cul3, cul3a, CUL-3-A, MGC79108, Cullin-3-A, cul3b, CUL-3-B, MGC80402, Cullin-3-B, AI467304, AW146203, mKIAA0617, MGC189292, wu:fc37e01, CUL3, ARABIDOPSIS THALIANA CULLIN 3, ARABIDOPSIS THALIANA CULLIN 3A, ATCUL3, ATCUL3A, CUL3A, CULLIN 3, CULLIN 3A, T24P13.25

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear Other Applications: Immunoprecipitation

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human Cullin-3 containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Apoptosis/Necrosis, Proteasome

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with Cullin-3 antibody