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Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) antibody

Antigen

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2)
Synonyms GAD65, MGC161605, MGC161607, Gad-2, GAD(65), 6330404F12Rik, gad65, zgc:112198, GAD2, F12P19.12, F12P19_12, GLUTAMATE DECARBOXYLASE 2
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125594
Quantity 500µl  (100ug/ml approximately)  (Variants)
Price 313.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name Glutamate Decarboxylase 65 (GAD65)
Immunogen A synthetic peptide derived from the C-terminus region of the human GAD65 protein.
Format Ig-fraction, Purified
Specificity This antibody reacts with a 65 kD protein, known as GAD (Glutamate Decarboxylase). Two forms of GAD exist in adults, GAD65 and GAD67, which are encoded by different genes. GAD65 and GAD67 are expressed in most GABA containing neurons. GAD65 appears to be targeted to the membrane and nerve ending, where as GAD67 is more widely distributed throughout the cell. This antibody cross reacts with rat.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreas. Cellular localization: Cytoplasmic
Concentration 100ug/ml approximately
Buffer Purified immunoglobulin fraction of rabbit antiserum against human GAD65 containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Enzymes, Metabolism
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2)", type "Antibodies"
Hosts Rabbit (53), Goat (5), Mouse (2)
Reactivities Human (56), Rat (Rattus) (47), Mouse (Murine) (41), Pig (Porcine) (32), Chicken (30), Cow (Bovine) (20), Dog (Canine) (20), Horse (Equine) (2), Chimpanzee (1), Monkey (1), Rabbit (1), Sheep (Ovine) (1)
Applications Immunofluorescence (IF) (39), Flow Cytometry (FACS) (36), Western Blotting (WB) (19), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Enzyme Immunoassay (EIA) (3), Immunoelectron Microscopy (IEM) (2), Radioimmunoassay (RIA) (2), Immunocytochemistry (ICC) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (IHC) (1)
Conjugates Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)