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Antibodies
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anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) ant...
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) antibody
| Antigen |
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| Synonyms |
Flk1, Ly73, Flk-1, Krd-1, VEGFR2, VEGFR-2, sVEGFR-2, 6130401C07, FLK1, CD309, VEGFR, Vegfr-2, MGC93590, KDR, FLK-1, flk-1, vegfr-2 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN125595 |
| Quantity |
500µl (100ug/ml approximately) (Variants) |
| Price |
313.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Flk-1 (Flk-1/KDR/VEGFR2)
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Immunogen
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A synthetic peptide derived from the C-terminus of the precursor form of the mouse Flk-1.
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with a 180 kD protein, known as Flk-1/KDR/Vascular endothelial growth factor receptor 2. Flk-1 is a cell membrane receptor kinase. Flk-1 is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. This antibody cross reacts with mouse and rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Angiosarcoma. Cellular localization: Cytoplasmic Other Applications: Western Blotting
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against human Flk-1 containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cytokines, Receptors
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR)", type "Antibodies"
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Hosts
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Rabbit (111), Mouse (9), Chicken (1), Escherichia Coli (E. coli) (1), Rat (1)
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Reactivities
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Human (120), Mouse (Murine) (88), Rat (Rattus) (74)
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Applications
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Western Blotting (WB) (75), Immunohistochemistry (IHC) (54), Immunofluorescence (IF) (53), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (42), Enzyme Immunoassay (EIA) (19), ELISA (16), Flow Cytometry (FACS) (11), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunoprecipitation (IP) (6), Functional Studies (Func) (4)
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Conjugates
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Biotin (5)
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Epitopes
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pTyr1175 (9), pTyr1214 (9), pTyr951 (9), Ab-1175 (5), Ab-1214 (5), Ab-951 (5), C-Term (3), N-Term (3), pY1054 (2), pTyr1054 (1), pTyr1059 (1), pTyr996 (1), pY951 (1), pY996 (1)
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