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Antibodies
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anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Pe...
Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) antibody
| Antigen |
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| Synonyms |
flt1b, VEGFR1, FLT, Flt-1, sFlt1, VEGFR-1, AI323757, FLT1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Immunofluorescence (IF) (26), Western Blotting (WB) (24), Flow Cytometry (FACS) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunoprecipitation (IP) (14), Enzyme Immunoassay (EIA) (13), ELISA (11), Immunohistochemistry (IHC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN125596 |
| Quantity |
500µl (100ug/ml approximately) (Variants) |
| Price |
313.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Flt-1 (Flt-1/VEGFR1)
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Immunogen
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A synthetic peptide derived from the C-terminus of the Flt-1 precursor of human origin.
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with a 180 kD protein, known as Flt-1/Vascular endothelial growth factor receptor 1. Flt-1 is a cell membrane receptor kinase. Flk-1 has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. This antibody cross reacts with mouse and rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Angiosarcoma. Cellular localization: Cytoplasmic Other Applications: Western Blotting
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against human Flt-1 containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cytokines, Receptors
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1)", type "Antibodies"
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Hosts
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Rabbit (44), Mouse (15), Chicken (1)
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Reactivities
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Human (60), Mouse (Murine) (38), Rat (Rattus) (30), Rabbit (3), Cow (Bovine) (2), Dog (Canine) (2), Pig (Porcine) (2), Chicken (1), Guinea Pig (1), Horse (Equine) (1)
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Applications
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Immunofluorescence (IF) (26), Western Blotting (WB) (24), Flow Cytometry (FACS) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunoprecipitation (IP) (14), Enzyme Immunoassay (EIA) (13), ELISA (11), Immunohistochemistry (IHC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Biotin (5), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (6), N-Term (3), pTyr1333 (2), pTyr1048 (1)
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