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Antibodies
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anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3...
X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3) antibody
| Antigen |
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| Synonyms |
AI182522, AW537713, 4432412E01Rik, MGC69118, im:7142103, zgc:101608, si:dkey-11b8.1, xrcc3, XRCC3, MGC142843 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Western Blotting (WB) (37), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), ELISA (7), ELISA (Detection) (3), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN125602 |
| Quantity |
500µl (100ug/ml approximately) (Variants) |
| Price |
313.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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X-Ray Repair Cross Complementing (XRCC3)
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Immunogen
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A synthetic peptide derived from the N-terminus of human XRCC3 protein.
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with a 32 kDa protein, known as XRCC3 (X-Ray Cross Complementing 3). XRCC3 is essential for repairing the double strand DNA breaks by homologous recombination between sister chromatids. It is homologous to Rad1. Five human homologues of RAD 51, XRCC2, XRCC3, RAD51B, RAD51C, RAD51D, interact among themselves to form one big complex or several smaller complexes.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Testis. Cellular localization: Nuclear, cytoplasmic Other Applications: Western Blotting (1:100-500).
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against human XRCC3 containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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DNA/RNA
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3)", type "Antibodies"
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Hosts
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Rabbit (46), Mouse (10)
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Reactivities
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Human (52), Mouse (Murine) (18), Rat (Rattus) (3)
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Applications
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Western Blotting (WB) (37), Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), ELISA (7), ELISA (Detection) (3), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (IHC) (3), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Biotin (3), HRP (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Center (2)
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