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Antibodies
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anti-TIMP Metallopeptidase Inhibitor 2 (TIMP2) antibody
TIMP Metallopeptidase Inhibitor 2 (TIMP2) antibody
| Antigen |
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| Synonyms |
CSC-21K, MGC84288, MGC108274, TIMP-2, TIMP2, timp2 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (90), Mouse (Murine) (34), Rat (Rattus) (29), Rabbit (25), Cow (Bovine) (21), Pig (Porcine) (21), Guinea Pig (5), Chicken (4), Dog (Canine) (2), Monkey (2), Zebrafish (Danio rerio) (2)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (72), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (40), Immunofluorescence (IF) (36), Flow Cytometry (FACS) (23), ELISA (17), Immunoprecipitation (IP) (17), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunohistochemistry (IHC) (5), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125611 |
| Quantity |
500µl (100ug/ml approximately) (Variants) |
| Price |
295.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Tissue Inhibitor of Metalloproteinase-2 (TIMP-2)
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Immunogen
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A synthetic peptide from the N-terminal half of human TIMP-2 protein.
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with a 21kD protein. The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of matrix metalloproteinases (MMPs) and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent and active forms of Type IV collagenase (MMP-2). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against TIMP-2 containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cancer, Extracellular Matrix, Inflammation
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "TIMP Metallopeptidase Inhibitor 2 (TIMP2)", type "Antibodies"
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Hosts
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Mouse (54), Rabbit (40), Sheep (3), Goat (1)
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Reactivities
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Human (90), Mouse (Murine) (34), Rat (Rattus) (29), Rabbit (25), Cow (Bovine) (21), Pig (Porcine) (21), Guinea Pig (5), Chicken (4), Dog (Canine) (2), Monkey (2), Zebrafish (Danio rerio) (2)
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Applications
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Western Blotting (WB) (72), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (40), Immunofluorescence (IF) (36), Flow Cytometry (FACS) (23), ELISA (17), Immunoprecipitation (IP) (17), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunohistochemistry (IHC) (5), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (7), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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N-Term (2)
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