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Antibodies
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anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
| Antigen |
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| Synonyms |
KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125620 |
| Quantity |
500µl (100ug/ml approximately) (Variants) |
| Price |
249.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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p27
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Immunogen
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A synthetic peptide derived from the C-terminal region of human p27kip1 protein.
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with a 27 kD protein. p27 protein, also know as kinase inhibitory protein (Kip1), binds to cyclin E/cdk2 complexes. p27kip1 is a candidate tumor suppressor gene and it functions as a negative regulator of G1 progression. p27kip1 has been proposed to function as a possible mediator of TGF-b induced G1 arrest. This antibody crossreacts with mouse and rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon Carcinoma. Cellular localization: Nuclear
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against p27kip1 containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cancer, Cell Cycle
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)", type "Antibodies"
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Hosts
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Rabbit (35), Mouse (7), Goat (4)
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Reactivities
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Human (42), Mouse (Murine) (21), Rat (Rattus) (19), Dog (Canine) (3), Cat (Feline) (1), Monkey (1)
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Applications
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Western Blotting (WB) (35), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), Immunoprecipitation (IP) (10), ELISA (9), Immunohistochemistry (IHC) (9), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Dot Blot (Dot) (1), Immunofluorescence (IF) (1)
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Epitopes
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pThr187 (4), internal (2), C-Term (1), T198 (1), pS10 (1), pS178 (1), pSer178 (1), pT157 (1), pT187 (1), pThr157 (1), pThr198 (1)
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