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Tumor Protein P53 (TP53) antibody

Antigen

Tumor Protein P53 (TP53)
Synonyms p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Catalog no. ABIN125624
Quantity 500µl  (100ug/ml approximately)  (Variants)
Price 158.00 $   Plus shipping costs $35.00
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anti-Tumor Protein P53 (TP53) antibody

Additional Information
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Alternative name P53
Immunogen Recombinant full-length wild type p53 protein.
Format Ig-fraction, Purified
Specificity This antibody reacts with a 53 kD protein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types. It is involved in regulating cell growth, replication and apoptosis. It binds to mdm2, SV40 T antigen and human Papilloma virus E6 protein. Mutation involving p53 is found in wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung and melanoma. This antibody crossreacts with mouse and rat.

Application Details
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Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Nuclear
Concentration 100ug/ml approximately
Buffer Purified immunoglobulin fraction of rabbit antiserum against p53 containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Transcription Factors
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
Hosts Rabbit (254), Mouse (80), Sheep (3)
Reactivities Human (328), Mouse (Murine) (67), Rat (Rattus) (61), Monkey (5), Cat (Feline) (3), Cow (Bovine) (3), Horse (Equine) (3), Dog (Canine) (2), Hamster (2), Primate (2), Primates (2), Sheep (Ovine) (2), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (279), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (85), Immunohistochemistry (IHC) (81), ELISA (68), Immunofluorescence (IF) (61), Immunoprecipitation (IP) (41), Enzyme Immunoassay (EIA) (40), Flow Cytometry (FACS) (27), Immunohistochemistry (Frozen Sections) (IHC (fro)) (26), Immunocytochemistry (ICC) (11), Dot Blot (Dot) (8), Transfected cell culture (TCC) (3), Radioimmunoassay (RIA) (2), Chromatin Immunoprecipitation (ChiP) (1)
Conjugates FITC (7), Biotin (2), HRP (1), PE (1)
Epitopes pSer37 (11), pSer315 (10), pSer33 (9), pSer46 (9), pThr18 (9), pSer392 (8), pSer6 (8), pSer15 (7), AA 371-380 (5), pSer20 (5), pSer32/Ser36 (5), AA 20-25 (4), pSer1232 (4), AA 18-30 (3), C-Term (3), S315 (2), S37 (2), S46 (2), T55 (2), pSer9 (2), AA 16-30 (1), AA 378-393 (1), AA 46-53 (1), AA 46-55 (1), Acetyl-Lys317 (1), Acetyl-Lys379 (1), S15 (1), S376 (1), S378 (1), S9 (1), T18 (1), amino acids 211-220 (1), pS20 (1), pS33 (1), pSer376 (1), pSer378 (1), pThr55 (1), phospho (1)