Cytokeratin 8 (KRT8) antibody
| Antigen |
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| Synonyms |
K8, KO, CK8, CYK8, K2C8, CARD2, K18, CYK18, Card2, EndoA, Krt2-8, Krt-2.8, AA960620, AL022697, AU019895, MGC118110, KRT2-8 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125627 |
| Quantity |
500µl (100ug/ml approximately) (Variants) |
| Price |
178.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Cytokeratin 8
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Immunogen
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Recombinant protein encoding human cytokeratin 8 (1-483 aa).
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with a 52.5 kD protein. Cytokeratin 8 belongs to type B (basic) subfamily of high molecular weight cytokeratins and exist in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas.It is absent in squamous cell carcinomas.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Skin, colon, lung. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against Cytokeratin 8 containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Cytokeratin 8 (KRT8)", type "Antibodies"
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Hosts
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Rabbit (40), Mouse (36), Guinea Pig (1)
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Reactivities
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Human (66), Rat (Rattus) (12), Mouse (Murine) (11), Pig (Porcine) (7), Cow (Bovine) (6), Sheep (Ovine) (6), Rabbit (5), Hamster (3), None (3), Mammalian (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (46), Western Blotting (WB) (46), Immunofluorescence (IF) (25), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Immunohistochemistry (IHC) (17), Enzyme Immunoassay (EIA) (6), Immunoprecipitation (IP) (6), ELISA (4), Immunocytochemistry (ICC) (4), Flow Cytometry (FACS) (1)
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Conjugates
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Biotin (2), FITC (1), HRP (1)
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Epitopes
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C-Term (4), pSer74 (4), pSer73 (1)
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