CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody

Antigen: CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E)

Synonyms: T3E, TCRE, FLJ18683, CD3E, cd3e, CD3, t3e, tcre

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125631

Additional Information

Alternative name: CD3 epsilon

Immunogen: A synthetic 13-mer peptide corresponding to aa 156-168 of the epsilon chain of human CD3 protein.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 20 kD protein. This antibody reacts with intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody cross reacts with baboon, monkey, horse, dog and cat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against CD3 containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: CD Antigens

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union