Vascular Endothelial Cell Growth Factor (VEGF) antibody

Antigen: Vascular Endothelial Cell Growth Factor (VEGF)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125636

Additional Information

Immunogen: A synthetic peptide from N-terminus of human VEGF.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 19-22 kD (reduced) protein. Vascular endothelial growth factor (VEGF) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. This antibody reacts with 165, 189 and 121 amino acid splice variants of VEGF of human, to a lesser extent, mouse and rat.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Hemangiosarcoma. Cellular localization: Cytoplasmic, cell membrane, extracellular matrix

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against VEGF containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union