Topoisomerase (DNA) II beta 180kDa (TOP2B) antibody

Antigen: Topoisomerase (DNA) II beta 180kDa (TOP2B)

Synonyms: TOPIIB, top2beta, TOP2B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125639

Additional Information

Alternative name: Topoisomerase II-beta

Immunogen: Recombinant protein encoding c-terminal 300 aa of human Topoisomerase II beta.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 300 kD protein. Topoisomerase II beta plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is unregulated in tumors and proliferating cells. Topoisomerase II beta is also implicated in drug resistance of tumor cells.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against Topoisomerase II beta containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: DNA/RNA, Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union