Proliferating Cell Nuclear Antigen (PCNA) antibody

Antigen: Proliferating Cell Nuclear Antigen (PCNA)

Synonyms: MGC8367, PCNAR, Pcna/cyclin, pcna

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN125640

Additional Information

Immunogen: Recombinant human PCNA protein.

Format: Ig-fraction

Specificity: This antibody reacts with a 36 kD protein. Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nuclei during late G1 phase immediately before the onset of DNA synthesis, becomes maximal during S-phase and declines during G2 and M phases. PCNA may act as an auxiliary protein of DNA polymerase-delta to play a functional role in the initiation of cell proliferation.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against PCNA containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Proliferation Markers

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union