Bcl-X antibody

Antigen: Bcl-X

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125641

Additional Information

Immunogen: A synthetic peptide corresponding to aa 46-66 of the human Bcl-X protein.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a ~27 kD protein. Bcl-X is a member of the bcl-2 family of proteins, which function as regulators of apoptosis. Bcl-X has two isomers, Bcl-XL (long) a 241 amino acids protein and Bcl-Xs (short) a 178 amino acid protein lacking 63 amino acid domain that is well conserved among members of the bcl-2 family. This antibody crossreacts with mouse.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Hodgkins lymphoma. Cellular localization: Cytoplasmic, cell/nuclear membrane

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against Bcl-X containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union