tyrosinase (Oculocutaneous Albinism IA) (TYR) antibody

Antigen: tyrosinase (Oculocutaneous Albinism IA) (TYR)

Synonyms: OCA1A, OCAIA, SHEP3, c, skc35, albino, sandy, zgc:109705, Tyr, C, TYR, tyr, TYRO, LOC100136546

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125647

Additional Information

Alternative name: Tyrosinase

Immunogen: Recombinant human tyrosinase protein.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with 70-80 kD protein. Tyrosinase is a copper containing metalloglycoprotein that catalyzes several step in the melanin pigment biosynthetic pathway. Tyrosinase and melan A are the most commonly expressed melanoma markers and are found in approximately 80 to 100% of cases. This antibody is helpful in the identification of melanocytic tumors.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against tyrosinase containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union