Cadherin antibody
| Antigen |
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| Synonyms |
PCDH10, OLpcad, cadherin, ci-cadherin |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Human, Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine), Monkey, Xenopus laevis
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Application
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Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN125648 |
| Quantity |
500µl (100ug/ml approximately) (Variants) |
| Price |
316.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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A synthetic peptide from the c-terminus of chicken N-cadherin.
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with 120 kD protein. Cadherins are calcium dependent cell adhesion molecules, which play important role in the growth and development of cells. In adhesion junctions, cadherins are bound to beta and gamma catenins, which in turn bind to alpha catenin. Cadherins play important role in tumor invasion and metastasis. This antibody crossreacts with mouse, rat, chicken, dog, monkey and xenopus.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against pan cadherin containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Extracellular Matrix, Signaling, Cytoskeleton
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Cadherin", type "Antibodies"
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Hosts
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Rabbit (14), Mouse (4)
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Reactivities
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Chicken (9), Human (9), Mouse (Murine) (3), Mammalian (2), Dog (Canine) (1), Monkey (1), Rat (Rattus) (1), Xenopus laevis (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Western Blotting (WB) (7), Immunohistochemistry (IHC) (5), Immunoprecipitation (IP) (2), ELISA (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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