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Antibodies
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anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
| Antigen |
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| Synonyms |
ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, zERK1, fi06b09, wu:fi06b09, Tb08.10J17.940 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (134), Rat (Rattus) (69), Mouse (Murine) (68), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Primate (2), Rabbit (2), Dog (Canine) (1), Zebrafish (Danio rerio) (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (123), ELISA (34), Immunofluorescence (IF) (32), Enzyme Immunoassay (EIA) (30), Immunohistochemistry (IHC) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunoprecipitation (IP) (17), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
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| Catalog no. |
ABIN125654 |
| Quantity |
500µl (100ug/ml approximately) (Variants) |
| Price |
250.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Extracellular Signal-Regulated Kinase-1 (ERK1)
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Immunogen
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A synthetic peptide derived from C-terminus of rat ERK-1 protein.
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Format
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Ig-fraction
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Specificity
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Erk1 / extracellular signal-regulated kinase 1 / p44 MAP kinase / microtubule-associated protein 2 kinase / myelin basic protein kinase / EGF receptor T669 kinase is ubiquitously expressed in various tissues and cells. It gets activated by phosphorylation of neighboring serine and threonine residues by Mek 1 and Mek2 in response to diverse stimuli like growth factors and mitogens. This antibody cross reacts with mouse and rat.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against ERK-1 containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Kinases/Phosphatases
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Mitogen-Activated Protein Kinase 3 (MAPK3)", type "Antibodies"
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Hosts
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Rabbit (104), Mouse (28), Goat (6), Chicken (1), Sheep (1)
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Reactivities
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Human (134), Rat (Rattus) (69), Mouse (Murine) (68), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Primate (2), Rabbit (2), Dog (Canine) (1), Zebrafish (Danio rerio) (1)
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Applications
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Western Blotting (WB) (123), ELISA (34), Immunofluorescence (IF) (32), Enzyme Immunoassay (EIA) (30), Immunohistochemistry (IHC) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunoprecipitation (IP) (17), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
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Conjugates
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Biotin (1), HRP (1)
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Epitopes
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N-Term (7), pThr202 (6), pTyr204 (6), pThr202/pTyr204 (5), C-Term (4), pThr202/Tyr204 (2), D192 (1), T202 (1), Y204 (1), pT202 (1), pY204 (1)
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