Fragile Histidine Triad Gene (FHIT) antibody

Antigen: Fragile Histidine Triad Gene (FHIT)

Synonyms: FHIT, MGC73176, zgc:73176, Fra14A2, AW045638, fhit, MGC89657, LOC100223655, FRA3B, AP3Aase

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125658

Additional Information

Alternative name: FHIT (Fragile Histidine Triad)

Immunogen: A recombinant full length human FHI protein.

Format: Ig-fraction

Specificity: This antibody reacts with human FHIT (Fragile Histidine Triad). Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach, and pancreas. In normal cells, FHIT may act as a tumor suppressor.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against FHIT protein containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast stained with FHIT antibody