Integrin alpha-L (ITGAL) (alpha subunit) antibody

Details for Product No. ABIN125682
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Antigen
Synonyms CD11A, LFA-1, LFA1A, (p180), Cd11a, LFA-1A, Ly-15, Ly-21, CD11a
Epitope
alpha subunit
(4), (2), (1)
Reactivity
Human
(206), (165), (49), (35), (27), (25), (11), (6), (2), (1), (1), (1)
Host
Mouse
(265), (133), (28), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(86), (51), (48), (15), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Functional Studies (Func), Immunoprecipitation (IP), Flow Cytometry (FACS)
(359), (119), (64), (52), (50), (35), (24), (16), (15), (15), (10), (8), (8), (5), (3), (1), (1), (1)
Pubmed 7 references available
Catalog no. ABIN125682
Quantity 0.1 mg
Price
290.40 $   Plus shipping costs $45.00
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Immunogen Leukocytes from a pacient suffering from a LGL-type leukaemia.
Clone MEM-25
Isotype IgG1
Specificity The antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. HLDA IV, WS Code NL 209
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 µm filtered
Alternative Name ITGAL
Background CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Research Area Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Recommended dilution: 2 µg/mL
Immunoprecipitation: excellent antibody for immunoaffinity purification of LFA-1 complex
Functional Application: The antibody MEM-25 partially blocks binding of LFA-1 complex to ICAM-1.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
General Ottonello, Epstein, Dapino et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).

Simon, Cherapanov, Nadra et al.: "Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2891-901, 1999 (PubMed).

Garnotel, Rittié, Poitevin et al.: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).

Hajishengallis, Martin, Sojar et al.: "Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 403-11, 2002 (PubMed).

Mathison, Befus, Davison et al.: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Wang, Kwas, Wu: "Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission." in: Journal of virology, Vol. 83, Issue 9, pp. 4195-204, 2009 (PubMed).

Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

Hosts (265), (133), (28), (4)
Reactivities (206), (165), (49), (35), (27), (25), (11), (6), (2), (1), (1), (1)
Applications (359), (119), (64), (52), (50), (35), (24), (16), (15), (15), (10), (8), (8), (5), (3), (1), (1), (1)
Conjugates (86), (51), (48), (15), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (1)
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