CD18 antibody

Antigen: CD18

Clonality: Monoclonal (MEM-48)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125687

Additional Information

Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.

Format: Azide Free

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM-48

Description: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule toconstitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2(CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18).In most cases, the response mediated by the integrin is a composite of the functions of itsindividual subunits. These integrins are essential for proper leukocyte migration, mediatingintercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severereduction of CD18 expression leads to the development of a psoriasiform skin disease.CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient tomediate leukotoxin-mediated cytolysis.

Specificity: The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-richrepeat 3 of the CD18 antigen (integrin beta2 subunit, beta2 integrin). CD18 is a 90-95 kDatype I transmembrane protein expressed on all leukocytes. This antibody does not react with: Dog (Canine).

Application Details

Application Notes: Western Blotting - Recommended dilution: 2 µg/ml. Positive control: Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml. Positive tissue: spleen, microgliaImmunohistochemistry (frozen sections). Functional Application: The antibody MEM-48 induces high-affinity conformation of LFA-1 complex.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48). Lane 1: KG-1a human acute myeloid leukemia cell line. Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line

Surface staining of human peripheral blood cells with anti-human CD18 (MEM-48) PE. Cells in the granulocyte gate were used for analysis.

Publications

Publications: Ottonello, Epstein, Dapino et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).

Garnotel, Rittiue, Poitevin et al.: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).

Nakayama, Yoshizaki, Prinetti et al.: "Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms." in: Journal of leukocyte biology, Vol. 83, Issue 3, pp. 728-41, 2008 (PubMed).

Sewald, Gebert-Vogl, Prassl et al.: "Integrin subunit CD18 Is the T-lymphocyte receptor for the Helicobacter pylori vacuolating cytotoxin." in: Cell host & microbe, Vol. 3, Issue 1, pp. 20-9, 2008 (PubMed).

Zemani, Silvestre, Fauvel-Lafeve et al.: "Ex vivo priming of endothelial progenitor cells with SDF-1 before transplantation could increase their proangiogenic potential." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 4, pp. 644-50, 2008 (PubMed).

Morova, Osicka, Masin et al.: "RTX cytotoxins recognize beta2 integrin receptors through N-linked oligosaccharides." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 14, pp. 5355-60, 2008 (PubMed).

Cera, Fabbri, Molendini et al.: "JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling." in: Journal of cell science, Vol. 122, Issue Pt 2, pp. 268-77, 2009 (PubMed).

Tran, Glass, Uzel et al.: "Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 5, pp. 2929-38, 2009 (PubMed).

Balashova, Shah, Patel et al.: "Aggregatibacter actinomycetemcomitans LtxC is required for leukotoxin activity and initial interaction between toxin and host cells." in: Gene, Vol. 443, Issue 1-2, pp. 42-7, 2009 (PubMed).