CD43 antibody

Antigen: CD43

Clonality: Monoclonal (MEM-59)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN125693

Additional Information

Immunogen: Human T lymphocytes.

Format: Azide Free

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM-59

Description: CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negativecharge, expressed on the surface of most hematopoietic cells. CD43 contributes to arepulsive barrier that interferes with cellular adhesion, however, in certain cases alsopromotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesinCD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cellinteractions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesivenessand counteracts LFA-1 induction via other receptors. Expression of CD43 causes inductionof functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiencyCD43 promotes tumour proliferation and viability. It appears to be an important modulator ofleukocyte functions.

Specificity: The antibody MEM-59 recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), a95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyteactivation. CD43 is expressed by platelets and at high levels on the surface of allleukocytes, it is negative on resting B lymphocytes and erythrocytes.HLDA IV, WS Code NL 604HLDA V, WS Code AS S290

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Positive tissue: thymus, tonsil. Functional Application: The antibody MEM-59 activates T lymphocytes and also induces apoptosis in hemopoieticprogenitor cells.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only