CD58 (CD58) antibody
| Antigen |
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| Synonyms |
LFA3, LFA-3, FLJ23181, FLJ43722 |
| Clonality |
Monoclonal (MEM-63) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)
Flow Cytometry (FACS) (68), Immunoprecipitation (IP) (26), Western Blotting (WB) (21), Functional Studies (Func) (15), Immunohistochemistry (IHC) (9), Immunofluorescence (IF) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Cytotoxicity Test (CyTox) (4), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), ELISA (2), ELISA (Detection) (2)
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| Catalog no. |
ABIN125698 |
| Quantity |
0.1 mg (1 mg/ml) (Variants) |
| Price |
260.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD58
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Immunogen
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NALM-6 human pre-B cell line
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Format
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Azide Free
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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MEM-63
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Description
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CD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by bothhematopoietic and non-hematopoietic cells (often on antigen presenting cells) and servingas ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 isexpressed in transmembrane form and in GPI-anchored form, the later is constitutivelyassociated with protein kinases whereas the transmembrane form activates kinase activityupon triggering. CD58 is a powerful tool for detection of minimal residual disease in acutelymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.
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Specificity
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The antibody MEM-63 reacts with CD58 (LFA-3), a 40-70 kDa glycoprotein distributed overmany tissues, leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.HLDA VI, WS Code AS A047
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Application Notes
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Western Blotting. Application note: Non-reducing conditions. Functional Application: The antibody MEM-63 inhibits CD2/CD58 interactions.
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from hybridoma culture supernatant by protein-A affinity chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 ยต m filter sterilized.
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "CD58 (CD58)", type "Antibodies"
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Hosts
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Mouse (89), Rabbit (1)
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Reactivities
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Human (79), Sheep (Ovine) (14), Pig (Porcine) (11)
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Applications
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Flow Cytometry (FACS) (68), Immunoprecipitation (IP) (26), Western Blotting (WB) (21), Functional Studies (Func) (15), Immunohistochemistry (IHC) (9), Immunofluorescence (IF) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Cytotoxicity Test (CyTox) (4), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), ELISA (2), ELISA (Detection) (2)
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Conjugates
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Biotin (13), FITC (13), PE (7), RPE (4), APC (2), Alexa Fluor 488 (1), Cy5,PE (1), Pacific Blue (1)
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