Tubulin, alpha (TUBA) antibody (Dyomics 547)

Antigen: Tubulin, alpha (TUBA)

Synonyms: Tuba1, LOC397931, MGC82007, Tua1, bmtua1, Tua2, bmtua2, Tua3, bmtua3, ALPHA TUBULIN, T29J13.200, TUA5, K-ALPHA-1, 4t, ALPHA 67C, D.m.ALPHA-67C, DTA2, Fs(3)Kav, Fs(3)Kavar, Fs(3)Sz14, Fs(3)Sz22, Fs(3)Tom, Fs(3)Tomaj, Kavar, T, Tom, Tub, aTub67C, alpha-Tub, alpha-Tub67C, alpha-tubulin, alpha-tubulin[4], alpha4, alpha4-t, alpha4-tubulin, alpha4Tub67C, alpha4t, alpha67C, alphaTUB, alphaTub4, alphaTub[67C], alphatub67C, mum-tub67C, DmelCG8308, CG8308, 1t, ALPHA 84C, BEST:LD32507, DM1alpha, DTA1, Talpha1, Tuba84B, Tubalpha1, a1t, aTub84B, agr;Tub, alpha-Tub84B, alpha1, alpha1t, alpha1tub, alpha84B, alphaT, alphaTub1, alphat-1, anon-EST:Liang-1.59, anon-EST:Liang-2.30, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tubulin, DmelCG1913, CG1913, 3t, ALPHA 84D, D.m.ALPHA-84D, DTA4, aTub84D, alpha-Tub84D, alpha3, alpha3t, alpha84D, alphaTub3, DmelCG2512, CG2512, 2t, ALPHA 85E, DTA3, a2t, aTub85E, alpha-Tub85E, alpha2, alpha2t, alpha85E, alpha85E-tub, alphaTub2, alphaTub84E, DmelCG9476, CG9476, TUBA3E, TUBA2, LIS3, TUBA3, FLJ25113, B-ALPHA-1, Tuba3b, Tuba3a, RGD1565155, TUBA3D, MGC133894, tua3

Clonality: Monoclonal (TU-01)

Host: Mouse

Reactivity: Various Species

Conjugate: Dyomics 547

Catalog no.: ABIN125703

Additional Information

Alternative name: alpha-tubulin

Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization -depolymerization.

Isotype: IgG1

Clone: TU-01

Description: The microtubules are intracellular dynamic polymers made up of evolutionarily conservedpolymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associatedproteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamicminus ends. Microtubules are required for vital processes in eukaryotic cells includingmitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are alsonecessary for movement of cells by means of flagella and cilia. In mammalian tissue culturecells microtubules have their minus ends anchored in microtubule organizing centers(MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimerto associate with other heterodimers to form microtubule. In vivo, microtubule dynamics varyconsiderably. Microtubule polymerization is reversible and a populations of microtubules incells are on their minus ends either growing or shortening this phenomenon is calleddynamic instability of microtubules. On a practical level, microtubules can easily bestabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or morecommonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at roomtemperature for many hours. Using limited proteolysis by enzymes both tubulin subunits canbe divided into N-terminal and C-terminal structural domains.The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that existsin cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. Indifferent species it is coded by multiple tubulin genes that form tubulin classes (in human 6genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypesare expressed ubiquitously, while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypesand their posttranslational modifications are responsible for multiple tubulin charge variants -tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structuraldomain.

Specificity: The antibody TU-01 recognizes the defined epitope (aa 65-97) on N-terminal structuraldomain of alpha-tubulin.

Synonyms: Tuba1, LOC397931, MGC82007, Tua1, bmtua1, Tua2, bmtua2, Tua3, bmtua3, ALPHA TUBULIN, T29J13.200, TUA5, K-ALPHA-1, 4t, ALPHA 67C, D.m.ALPHA-67C, DTA2, Fs(3)Kav, Fs(3)Kavar, Fs(3)Sz14, Fs(3)Sz22, Fs(3)Tom, Fs(3)Tomaj, Kavar, T, Tom, Tub, aTub67C, alpha-Tub, alpha-Tub67C, alpha-tubulin, alpha-tubulin[4], alpha4, alpha4-t, alpha4-tubulin, alpha4Tub67C, alpha4t, alpha67C, alphaTUB, alphaTub4, alphaTub[67C], alphatub67C, mum-tub67C, DmelCG8308, CG8308, 1t, ALPHA 84C, BEST:LD32507, DM1alpha, DTA1, Talpha1, Tuba84B, Tubalpha1, a1t, aTub84B, agr;Tub, alpha-Tub84B, alpha1, alpha1t, alpha1tub, alpha84B, alphaT, alphaTub1, alphat-1, anon-EST:Liang-1.59, anon-EST:Liang-2.30, clone 1.59, clone 2.30, l(3)84Bd, l(3)g3, ms(3)nc33, nc33, tubulin, DmelCG1913, CG1913, 3t, ALPHA 84D, D.m.ALPHA-84D, DTA4, aTub84D, alpha-Tub84D, alpha3, alpha3t, alpha84D, alphaTub3, DmelCG2512, CG2512, 2t, ALPHA 85E, DTA3, a2t, aTub85E, alpha-Tub85E, alpha2, alpha2t, alpha85E, alpha85E-tub, alphaTub2, alphaTub84E, DmelCG9476, CG9476, TUBA3E, TUBA2, LIS3, TUBA3, FLJ25113, B-ALPHA-1, Tuba3b, Tuba3a, RGD1565155, TUBA3D, MGC133894, tua3

Application Details

Application Notes: Immunocytochemistry on fixed and permeabilized cells. Suggested working dilution is 1: 200.It is recommended that the user titrates the reagent for use in the particular testing system.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Cytoskeleton

Restrictions: For Research Use only

Images

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01, green) and anti-Vimentin (VI-01, cat. no. 11-254-C100, red). Nucleus is stained with DAPI (blue).

Publications

Publications: Smertenko, Blume, Viklickuy et al.: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, 1997 (PubMed).

Miyajima, Maruyama, Nonomura et al.: "TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression." in: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 383-7, 2009 (PubMed).

Lukas, Mazna, Valenta et al.: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." in: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, 2009 (PubMed).

Bon, Di Carlo, Folgiero et al.: "Negative regulation of beta4 integrin transcription by homeodomain-interacting protein kinase 2 and p53 impairs tumor progression." in: Cancer research, Vol. 69, Issue 14, pp. 5978-86, 2009 (PubMed).

Honys, R?nuak, Fecikovua et al.: "Cytoskeleton-associated large RNP complexes in tobacco male gametophyte (EPPs) are associated with ribosomes and are involved in protein synthesis, processing, and localization." in: Journal of proteome research, Vol. 8, Issue 4, pp. 2015-31, 2009 (PubMed).