CD18 antibody

Antigen: CD18

Clonality: Monoclonal (MEM-148)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)

Catalog no.: ABIN125713

Additional Information

Immunogen: Peripheral blood mononuclear cells

Format: Azide Free

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM-148

Description: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule toconstitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2(CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18).In most cases, the response mediated by the integrin is a composite of the functions of itsindividual subunits. These integrins are essential for proper leukocyte migration, mediatingintercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severereduction of CD18 expression leads to the development of a psoriasiform skin disease.CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient tomediate leukotoxin-mediated cytolysis.

Specificity: The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible inintact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa typeI transmembrane protein expressed on all leukocytes.HLDA VI, WS Code AS A052 This antibody does not react with: Porcine.

Application Details

Application Notes: Flow Cytometry: The antibody MEM-148 is excellent marker of activated myeloid cells. Application note: Non-reducing conditions. Functional Application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1conformation in resting leukocytes.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).