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Antibodies
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anti-Platelet Glycoprotein Ib alpha Chain (GP1BA) antibody
Platelet Glycoprotein Ib alpha Chain (GP1BA) antibody
| Antigen |
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| Synonyms |
BSS, GP1B, CD42B, MGC34595, CD42b-alpha, GPIba, GPIbalpha |
| Clonality |
Monoclonal (HIP1) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
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| Catalog no. |
ABIN125719 |
| Quantity |
0.1 mg (1 mg/ml) (Variants) |
| Price |
273.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD42b
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Immunogen
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Peripheral blood mononuclear cells of a patient suffering with CLL.
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Format
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Azide Free
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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HIP1
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Description
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CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-Vreceptor complex critical in the process of platelet-rich thrombus formation by tethering theplatelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed ata site of vascular injury, as well as to thrombin, coagulation factors XI and XII, highmolecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domainof CD42b by its interactions also contributes to metastasis.
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Specificity
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The antibody HIP1 reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoproteinexpressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composedin a disulfide linked heterodimer (CD42b/c, 160 kDa), CD42b/c forms a noncovalentcomplex with CD42a and CD42d.HLDA IV, WS Code P 40
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Application Notes
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Functional Application: The antibody HIP1 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF)to platelets and ristocetin-induced platelet agglutination.
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by protein-G affinity chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 ยต m filter sterilized.
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "Platelet Glycoprotein Ib alpha Chain (GP1BA)", type "Antibodies"
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Hosts
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Mouse (13)
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Reactivities
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Human (13), Primates (2)
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Applications
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Flow Cytometry (FACS) (11), Functional Studies (Func) (3), Immunofluorescence (IF) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoassay (IA) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Western Blotting (WB) (1)
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Conjugates
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Biotin (2), APC (1), FITC (1), PE (1), RPE (1), SPRD (1)
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